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Custom Synthetic DNA library synthesis services

The traditional method of “design-build-test” routine to reach a genetic design to improve synthetic biology has been proven to be slow, poor, and unreliable. Due to this reason, an alternative method of generating a DNA libraries was discovered and utilized within the research community with higher frequency. With the Synthetic DNA library synthesis service, high performing genetic solutions will be worked out with ease and efficiency. The DNA library synthesis pool can achieve gene synthesis and cloning with a much faster, smarter, and more reliable approach. This allows for a much more accurate and efficient DNA library to be synthesized with the use of DNA synthesis. With a more accurate and efficient DNA library, various topics within genetics research will better understood and more effectively studied.

Synthetic DNA library synthesis makes biological engineering much more efficient

With DNA library synthesis based methods, highly complex sequences within synthetic biology can be engineered rapidly with high accuracy. It is a novel approach which allows for the customer specific DNA sequence to be more reliably synthesized and obtained with ease and higher accuracy. This also enables new biological systems and products to move toward potential revolutionary discoveries.

DNA synthesis - an overview | ScienceDirect Topics

DNA synthesis in regenerating rat liver is complete by 72 hours

Typically, synthetic oligonucleotides aresingle-stranded DNA or RNA molecules around 15–25 bases in length.

Mechanism of 3'-dephosphorylation of oligonucleotidesassembled on universal solid supports.To make the solid support material suitable for oligonucleotidesynthesis, non-nucleosidic linkers or nucleoside succinates arecovalently attached to the reactive amino groups in AminopropylCPG, LCAA CPG, or Aminomethyl MPPS.

Mechanism of action of 5-fluorouracil (5-FU), which binds to and inhibits the enzyme thymidylate synthase (TS), thereby reducing DNA synthesis and cell proliferation and inducing cell death.

DNA Synthesis - an overview | ScienceDirect Topics

5-FU is a fluoropyrimidine that acts as an antimetabolite by binding to the enzyme thymidylate synthase, which is responsible for the synthesis of nucleotides. The resulting inhibition of thymidylate synthase leads to a reduction in DNA synthesis and cell proliferation, inducing cell death. These effects are particularly evident in cells with high mitotic rates, such as neoplastic cells. 5-FU is also incorporated into DNA or RNA, interfering with their normal functioning ().

DNA Synthesis Related Services

Synbio Technologies is building up the first integrated GPS (Genotype, Phenotype and Synotype) system aimed to a quick and easy translation or reverse translation between "Genotype" and "Phenotype" by using our proprietary "Synotype" platform. The company's scientific capabilities encompass areas such as DNA engineering, DNA synthesis, genome synthesis, pathway synthesis, synthetic biology, pharmacogenomics, microbiology, translational biology and the applications of synthetic biology. Synbio Technologies' team has a proven track record regarding translating scientific breakthroughs into cost effective biological solution.

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If a synthetic “donor” DNA containing the desired ..

Translesion DNA Synthesis in Cancer: Molecular …

allows us to synthesize gene libraries of any size and complexity, specific to the customer’s specifications. DNA library synthesis relies upon DNA synthesis, a method mastered by through our three phase Syno®Platform. This platform allows us to generate the customer specified DNA sequence of interest, up to and including 200kb in length, with one hundred percent accuracy. DNA synthesis is the technology that provides the foundation to meet the needs of the DNA library construction and maintenance. This strong foundation is important because DNA synthesis is the first step for generating DNA libraries. With the proper construction of DNA libraries researchers aim to identify novel genes and how each gene relates to various protein functions and structures.

A novel synthetic DNA minor groove binder ..

The synthesis of DNA from an RNA template, via reverse transcription, produces complementary DNA (cDNA). Reverse transcriptases (RTs) use an RNA template and a short primer complementary to the 3' end of the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for the Polymerase Chain Reaction (PCR). This combination of reverse transcription and PCR (RT-PCR) allows the detection of low abundance RNAs in a sample, and production of the corresponding cDNA, thereby facilitating the cloning of low copy genes. Alternatively, the first-strand cDNA can be made double-stranded using DNA Polymerase I and DNA Ligase. These reaction products can be used for direct cloning without amplification. In this case, RNase H activity, from either the RT or supplied exogenously, is required.

To initiate DNA synthesis, short primer sections of DNA …

Many RTs are available from commercial suppliers. and Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase are RTs that are commonly used in molecular biology workflows. lacks 3´ → 5´ exonuclease activity. is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild-type M-MuLV. The enzyme is active up to 50°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product, up to 12 kb in length.

Reverse Transcription (cDNA Synthesis) | NEB

Cytotoxic chemotherapy refers to agents whose mechanisms of action cause cell death or prevent cell growth, generally through inhibiting microtubule function, function, or synthesis. Cytotoxic chemotherapy mechanisms of action may be cell cycle-dependent—arresting cancer cell growth at specific phases in the cell cycle.

the DNA synthesis industry, and the synthetic biology ..

The ability to manipulate living organisms is at the heart of a range of emerging technologies that serve to address important and current problems in environment, energy, and health. However, with all its complexity and interconnectivity, biology has for many years been recalcitrant to engineering manipulations. The recent advances in synthesis, analysis, and modeling methods have finally provided the tools necessary to manipulate living systems in meaningful ways, and have led to the coining of a field named synthetic biology. The scope of synthetic biology is as complicated as life itself – encompassing many branches of science, and across many scales of application. New DNA synthesis and assembly techniques have made routine the customization of very large DNA molecules. This in turn has allowed the incorporation of multiple genes and pathways. By coupling these with techniques that allow for the modeling and design of protein functions, scientists have now gained the tools to create completely novel biological machineries. Even the ultimate biological machinery – a self-replicating organism – is being pursued at this moment. It is the purpose of this review to dissect and organize these various components of synthetic biology into a coherent picture.

Kinetic Mechanism of GTP Binding and RNA Synthesis …

The field of synthetic biology lies at the interface of many different biological research areas, such as functional genomics, protein engineering, chemical biology, metabolic engineering, systems biology, and bioinformatics. Not surprisingly, synthetic biology means different things to different people, even to leading practitioners in the field. To avoid possible confusion for the reader, here we would define it as “deliberate design of improved or novel biological systems that draws on principles elucidated by biologists, chemists, physicists, and engineers.” It is true that scientists have been attempting to design biological systems for decades. However, synthetic biology has become a field of its own only recently, mostly driven by the advances in systems biology and the development of new powerful tools for DNA synthesis and sequencing,. Synthetic biology has broad applications in medical, chemical, food, and agricultural industries. In addition to practical applications, synthetic biology also aims to increase our understanding of basic life sciences.

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